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Mycoplasma genitalium and Macrolide Resistance

S-DiaMGRes™

G-DiaPara
DDGS-65-L025

 Primers and probes
 Positive control
 H2O
 Internal control
 Master Mix

25 TESTS
Internal control suggested
DiaInhib™
Cy5

Mycoplasma genitalium is a small pathogenic bacterium which infects epithelial cells of the urinary and genital tracts. M. genitalium is an emerging sexually transmitted infection occuring during unprotected sexual intercourse. Mycoplasma genitalium is associated with notable reproductive tract syndromes such as cervicitis, pelvic inflammatory disease and infertility. Mycoplasma genitalium has been strongly associated with urethritis in both men and women and with cervicitis in women. Among Mycoplasma genitalium infected men, symptomatic urethritis is more common than asymptomatic urethritis. Owing to the slow cell replication and fastidious growth requirements, culture is impossible to use in clinical practice. Polymerase chain reaction (PCR) is currently the method of choice for detection.

Treatment using Azithromycin is often recommended for treating Mycoplasma genitalium infections but therapeutic failures have been reported in many cases of non-gonococcal urithritis caused by MG, because of the increased prevalence of resistance to macrolides. Resistance to macrolides of Mycoplasma genitalium is primarly down to mutations in the V region of the gene for 23S rRNA in nucleotides 2028 and/or 2059.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Mycoplasma genitalium ROX 591 nm MG 219 65 µL
    A2059 G, C Yellow dye 549 nm 23S rRNA 65 µL
    A2058 C, G, T FAM 520 nm 23S rRNA 65 µL
    Internal Control (IUC) Cy5 662 nm - 65 µL
    EXTRACTION METHODS
    Urine Swabs
    MagNA Pure 96 System (Roche)

    Recommended
    Suitable

    PCR instruments
    Roche ABI Bio-Rad
    LC 480 7500 Fast CFX96

    Recommended
    Suitable

  • Documents

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    Dec 16-Dec 17, 2019
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