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Respiratory panel

Resp'Easy™

Resp'easy
DDGR-90-L048
Pre-aliquoted strips x48
H2O
RNA master mix
DNA master mix
RNA extraction control virus
DNA extraction control virus
Positive control
Negative control
100 TESTS
Internal control suggested
EIC/DNApp
YD
EIC/RNApp
YD

This panel has been designed to detect 24 different major bacteria in respiratory infections :

Human influenza (HI) A/B, Human respiratory syncytial virus (RSV), Human coronavirus (HCOV), Human metapneumovirus (MPV), Human rhinovirus (HRV) and Human parainfluenza (HPIV) 1/2/3/4 are respiratory infections of upper and lower respiratory tracts causing bronchiolitis and pneumonia. Respiratory viral diseases account for approximately 200,000 hospitalizations during the flu season (November through to March) and an average of 23,600 deaths.1 These diseases most commonly affect infants and young children and can be fatal, especially in infants less than 1 year of age; adults may also be affected with the highest risk for disease severity and mortality among the elderly. 2 Among immune compromised patients respiratory viral disease can result
in increased heart and lung-related hospitalizations and other influenza-related complications, ultimately resulting in death.

Human adenoviruses are nonenveloped viruses with an icosahedral nucleocapsid containing a double stranded DNA genome. Adenoviruses are unusually stable to chemical or physical agents and adverse pH conditions, allowing for prolonged survival outside of the body and water. Adenoviruses are spread primarily via respiratory droplets. They are involved in multiple diseases like respiratory tract infections, conjunctivitis, keratoconjunctivitis, gastroenteritis. Most people recover from adenovirus infections by themselves, but people with immunodeficiency sometimes die of adenovirus infections.

Mycoplasma pneumoniae is a bacteria responsible for most of the cases of pneumonia in children. Transmission of Mycplasma pneumoniae can only occur through close contact and exchange of aerosols. Atypical pneumonia due to these bacteria usually causes milder forms of pneumonia and are characterized by a more protracted course of symptoms unlike other forms of pneumonia which can come on more quickly with more severe early symptoms. Mycoplasma pneumoniae can give rise to mild upper respiratory tract infection, bronchitis, bronchiolitis and bronchopneumonia. The disease usually starts as influenza-like syndrome with the predominant symptoms being fever, illness, headache, scratchy sore throat and cough. Transmission of M. pneumoniae can only occur through close contact and exchange of aerosols

Resp’EasyTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit allowing the qualitative detection of 24 respiratory pathogen subtypes and the differentiation of 12 respiratory pathogens by real-time PCR, in clinical specimens (nasopharyngeal swabs in GLY medium) from patients showing signs and symptoms of respiratory tract infection.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Adenovirus FAM 520 nm Hexon (H) A
    EIC/DNA Yellow dye 549 nm - A
    Mycoplasma pneumoniae FAM 520 nm Cytadhesin protein gene (P1) B
    Human coronavirus FAM 520 nm Replicase polyprotein (ORF1 ab) C
    Human rhinovirus FAM 520 nm Untranslated region (5’UTR) D
    EIC/RNA Yellow dye 549 nm - D
    Human parainfluenza 1 FAM 520 nm Polymerase (L) E
    Human parainfluenza 2 Yellow dye 549 nm Matrix protein E
    Human parainfluenza 3 FAM 520 nm Polymerase (L) F
    Human parainfluenza 4 Yellow dye 549 nm Nucleoprotein (NP) F
    Human metapneumovirus FAM 520 nm Nucleoprotein (NP) G
    Human respiratory syncytial virus Yellow dye 549 nm Nucleoprotein (NP) G
    Human influenza A FAM 520 nm Matrix protein 1 and 2 (MP1, MP2) H
    Human influenza B Yellow dye 549 nm Nucleoprotein (NP) H
    EXTRACTION METHODS
    Sputum Nasopharyngeal
    swabs/washes
    Bronchoalveolar
    Lavage
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad
    LC 480 7500 Fast CFX96
    DX
    Recommended
    Suitable
  • Documents
    SDS-DDGR-90-EN-v01-Resp'Easy en Download
    SDS-DDGR-90-FR-v01-Resp'Easy fr Download
    DDGR-90-L048_2016B24I  Download
  • Publications

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