Respiratory panel

Resp'Easy™

This panel has been designed to detect 24 different major bacteria and viruses in respiratory infections :

Human influenza (HI) A/B, Human respiratory syncytial virus (RSV), Human coronavirus (HCOV), Human metapneumovirus (MPV), Human rhinovirus (HRV) and Human parainfluenza (HPIV) 1/2/3/4 are respiratory infections of upper and lower respiratory tracts causing bronchiolitis and pneumonia. Respiratory viral diseases account for approximately 200,000 hospitalizations during the flu season (November through to March) and an average of 23,600 deaths.1 These diseases most commonly affect infants and young children and can be fatal, especially in infants less than 1 year of age; adults may also be affected with the highest risk for disease severity and mortality among the elderly. 2 Among immune compromised patients respiratory viral disease can result
in increased heart and lung-related hospitalizations and other influenza-related complications, ultimately resulting in death.

Human adenoviruses are nonenveloped viruses with an icosahedral nucleocapsid containing a double stranded DNA genome. Adenoviruses are unusually stable to chemical or physical agents and adverse pH conditions, allowing for prolonged survival outside of the body and water. Adenoviruses are spread primarily via respiratory droplets. They are involved in multiple diseases like respiratory tract infections, conjunctivitis, keratoconjunctivitis, gastroenteritis. Most people recover from adenovirus infections by themselves, but people with immunodeficiency sometimes die of adenovirus infections.

Mycoplasma pneumoniae is a bacteria responsible for most of the cases of pneumonia in children. Transmission of Mycplasma pneumoniae can only occur through close contact and exchange of aerosols. Atypical pneumonia due to these bacteria usually causes milder forms of pneumonia and are characterized by a more protracted course of symptoms unlike other forms of pneumonia which can come on more quickly with more severe early symptoms. Mycoplasma pneumoniae can give rise to mild upper respiratory tract infection, bronchitis, bronchiolitis and bronchopneumonia. The disease usually starts as influenza-like syndrome with the predominant symptoms being fever, illness, headache, scratchy sore throat and cough. Transmission of M. pneumoniae can only occur through close contact and exchange of aerosols

Resp’Easy^TM is a trusted and easy-to-use molecular diagnostic CE-IVD kit allowing the qualitative detection of 24 respiratory pathogen subtypes and the differentiation of 12 respiratory pathogens by real-time PCR, in clinical specimens (nasopharyngeal swabs in GLY medium) from patients showing signs and symptoms of respiratory tract infection.

• Validation

  DOUBLE-DYE PROBE & PRIMERS
  Targeted pathogens	Emission	Targeted genes	Volume
  Adenovirus	FAM 520 nm	Hexon (H)	A
  EIC/DNA	Yellow dye 549 nm	-	A
  Mycoplasma pneumoniae	FAM 520 nm	Cytadhesin protein gene (P1)	B
  Human coronavirus	FAM 520 nm	Replicase polyprotein (ORF1 ab)	C
  Human rhinovirus	FAM 520 nm	Untranslated region (5’UTR)	D
  EIC/RNA	Yellow dye 549 nm	-	D
  Human parainfluenza 1	FAM 520 nm	Polymerase (L)	E
  Human parainfluenza 2	Yellow dye 549 nm	Matrix protein	E
  Human parainfluenza 3	FAM 520 nm	Polymerase (L)	F
  Human parainfluenza 4	Yellow dye 549 nm	Nucleoprotein (NP)	F
  Human metapneumovirus	FAM 520 nm	Nucleoprotein (NP)	G
  Human respiratory syncytial virus	Yellow dye 549 nm	Nucleoprotein (NP)	G
  Human influenza A	FAM 520 nm	Matrix protein 1 and 2 (MP1, MP2)	H
  Human influenza B	Yellow dye 549 nm	Nucleoprotein (NP)	H

  EXTRACTION METHODS
  	Sputum	Nasopharyngeal swabs/washes	Bronchoalveolar Lavage
  NucleiSENS® EasyMAG® System (BioMérieux)
  MagNA Pure 96 System (Roche)
  QIAsymphony (Qiagen)

  Recommended
  Suitable

  PCR instruments
  Roche	ABI	Bio-Rad
  LC 480	7500 Fast	CFX96
  		DX

  Recommended
  Suitable

• Documents

  SDS	DDGR-90-L048	SDS-DDGR-90-EN-v01-Resp'Easy V01	EN	Download
  SDS	DDGR-90-L048	SDS-DDGR-90-FR-v01-Resp'Easy V01	FR	Download

  CoA	DDGR-90-L048	2016B24I		Download
  CoA	DDGR-90-L048	2018E18_I	2018-08-14	Download
  CoA	DDGR-90-L048	2017E08/I	2017-07-19	Download