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Legionella pneumophila / spp

R-DiaLegio™ (Available Soon )

flu RSV GL 8
DPSR-50-L080

Probes & Primers
H2O
Positive control
Negative control
Master Mix

80 TESTS
Internal control included
DiaControlDNA
Cy5

Legionella is a pathogenic group of Gram-negative bacteria that includes Legionella pneumophila, causing legionellosis and a mild flu-like illness called Pontiac fever. Legionella species typically exist in nature at low concentrations in groundwater, lakes and streams. The most common source of outbreaks are cooling towers primarily because of the risk for widespread circulation. Upon inhalation, the bacteria can infect alveolar macrophages, subverting the normal host cell machinery to create a niche where the bacteria can replicate. Legionella transmission is mostly via inhalation of water droplets. Once inside a host, incubation may take up to two weeks. First symptoms are flu-like, including fever, chills, and dry cough. Advanced stages of the disease cause problems with the gastrointestinal tract and the nervous system and lead to diarrhea and nausea.

R-DiaLegioTM will be validated on geneLEAD VIII and designed to qualitatively detect Legionella species and Legionella pneumophila in biological samples (bronchoalveolar lavage fluid, nasopharyngeal aspirates/swabs) collected from potentially infected patients using real-time PCR.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Legionella species FAM 520 nm L. species (16S) 320 µL
    Legionella pneumophila Yellow dye 549 nm Macrophage infectivity potentiator gene (MIP) 320 µL
    EXTRACTION METHODS
    Nasopharyngeal
    swabs/washes
    Bronchoalveolar
    Lavage
    geneLEAD VIII

    Recommended
    Suitable

    PCR instruments
    PSS
    geneLEAD VIII

    Recommended
    Suitable

  • Documents

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    Oct 9-Oct 11, 2019
    Noordwijk, the Netherlands
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    Dec 16-Dec 17, 2019
    Paris, France
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