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Legionella pneumophila / spp

R-DiaLeg™

R-DiaLeg
DDGR-50-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DiaControlDNA
DR TR Cy5 RD
DiaInhib™
DR Cy5

Legionella is a pathogenic group of Gram-negative bacteria that includes Legionella pneumophila, causing legionellosis and a mild flu-like illness called Pontiac fever. Legionella species typically exist in nature at low concentrations in groundwater, lakes and streams. The most common source of outbreaks are cooling towers primarily because of the risk for widespread circulation. Upon inhalation, the bacteria can infect alveolar macrophages, subverting the normal host cell machinery to create a niche where the bacteria can replicate. Legionella transmission is mostly via inhalation of water droplets. Once inside a host, incubation may take up to two weeks. First symptoms are flu-like, including fever, chills, and dry cough. Advanced stages of the disease cause problems with the gastrointestinal tract and the nervous system and lead to diarrhea and nausea.

R-DiaLegTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Legionella species and Legionella pneumophila in biological samples (bronchoalveolar lavage fluid, nasopharyngeal aspirates/swabs) collected from potentially infected patients using real-time PCR.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Legionella species FAM 520 nm L. species (16S) 250 µL
    Legionella pneumophila Yellow dye 549 nm Macrophage infectivity potentiator gene (MIP) 250 µL
    EXTRACTION METHODS
    Nasopharyngeal
    swabs/washes
    Bronchoalveolar
    Lavage
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAmp® DNA Mini Kit (Qiagen)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    Recommended
    Suitable
  • Documents
    SDS-DDGR-50-EN-v02-Legionella-pneumophila-species en Download
    SDS-DDGR-50-FR-v02-Legionella-pneumophila-species fr Download
    CoA_DDGR-50-L100_02006H  Download
  • Publications

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