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Human parainfluenza 1, 2, 3 and 4

R-DiaHPIV™

R-DiaHPIV
DDGR-84-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DRIC™
Cy5
DiaInhib™
DR Cy5

Human parainfluenza viruses (HPIV) are a group of four distinct single-stranded RNA viruses belonging to the Paramyxoviridae family. HPIVs consist of serotypes HPIV-1, HPIV-2, HPIV-3 and HPIV-4, belonging to two different genera: Respirovirus (HPIV-1, HPIV-3) and Rubulavirus (HPIV-2, HPIV-4). Most transmission have been linked to close contact, especially in nosocomial infections. HPIV-1 and 2 have been demonstrated to be the principal causative agents behind croup which is a viral disease of the upper airway. HPIV-3 has been closely associated with bronchiolitis and pneumonia and principally targets those aged less 1 year. HPIV-4 remains infrequently detected. All of these viruses spread readily within hospitals and can cause outbreaks in nurseries and pediatric wards. HPIVs remain the second main cause of hospitalisation in children under 5 years of age suffering from a respiratory illness.

R-DiaHPIVTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit allowing the qualitative detection by real-time PCR of human parainfluenza virus 1, 2, 3 and 4 in clinical specimens (nasopharyngeal swabs in GLY Medium) from patients potentially infected by these viruses.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Human parainfluenza 1 FAM 520 nm L gene (polymerase) 250 µL
    Human parainfluenza 2 Yellow dye 549 nm L gene (polymerase) 250 µL
    Human parainfluenza 3 FAM 520 nm M gene (matrix protein) 250 µL
    Human parainfluenza 4 Yellow dye 549 nm NP gene (Nucleoprotein) 250 µL
    EXTRACTION METHODS
    Sputum Nasopharyngeal
    swabs/washes
    Bronchoalveolar
    Lavage
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAmp® DNA Mini Kit (Qiagen)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    Flow DX
    Recommended
    Suitable
  • Documents
    SDS-DDGR-84-EN-v01-Human-Parainfluenza-Viruses-1-2-3-4 en Download
    SDS-DDGR-84-FR-v01-Virus-Parainfluenza-humains-1-2-3-4 fr Download
    CoA_DDGR-84-L100_04006I  Download
    CoA_DDGR-84-L100_16126A  Download
  • Publications

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