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Bordetella pertussis, Bordetella parapertussis

R-DiaBorM™

R-DiaBorM
DDGR-10-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DiaControlDNA
DR TR Cy5 RD
DiaInhib™
DR Cy5

Among the nine species of Bordetella identified to date, only four members (B. pertussis, B. parapertussis, B. holmesii, B. bronchiseptica) have been associated with respiratory infections in humans and others mammals. Bordetella is a Gram-negative encapsulated coccobacillus of the genus Bordetella, and the causative agent of the respiratory infection named pertussis or whooping cough. The infection occurs mostly in children under the age of one when they are unimmunized, or children with faded immunity, normally around the ages 11 through 18. The signs and symptoms are similar to a common cold: runny nose, sneezing, mild cough, and low-grade fever.

R-DiaBorMTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Bordetella pertussis & Bordetella parapertussis in biological samples (bronchoalveolar lavage fluid, sputum, nasopharyngeal specimens) collected from patients showing symptoms related to this pathogen using real-time PCR .

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Bordetella pertussis FAM 520 nm Insertion sequence (IS) 481 250 µL
    Bordetella parapertussis Yellow dye 549 nm Insertion sequence (IS) 1001 250 µL
    EXTRACTION METHODS
    Sputum Nasopharyngeal
    swabs/washes
    Bronchoalveolar
    Lavage
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAmp® DNA Mini Kit (Qiagen)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    DX
    Recommended
    Suitable
  • Documents
    SDS-DDGR-10-EN-v01-Bordetella-pertussis-parapertussis en Download
    SDS-DDGR-10-FR-v01-Bordetella-pertussis-parapertussis fr Download
    CoA_DDGR-10-L100_01006E  Download
    CoA_DDGR-10-L100_16267L 2017-03-06 Download
  • Publications

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