Shigella

G-DiaShig™

Shigella is a genus of Gram negative, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. Shigella species are classified by four serogroups (A, B, C and D). The causative agent of human shigellosis, Shigella causes disease in primates, but not in other mammals. Shigella is one of the leading bacterial causes of diarrhea worldwide, causing an estimated 80-165 million cases. Shigella infection is typically by ingestion. Shigella causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7. Both Shiga toxin and verotoxin are associated with hemolytic uremic syndrome.

G-DiaShig^TM is a trusted and easy-to-use molecular diagnostic CE-IVD kit allowing the qualitative detection of Shigella species (eg, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Shigella boydii) by real-time PCR in stool specimens obtained from patients with signs and symptoms of gastroenteritis.

• Validation

  DOUBLE-DYE PROBE & PRIMERS
  Targeted pathogens	Emission	Targeted genes	Volume
  Shigella	FAM 520 nm	ipaH	250 µL

  EXTRACTION METHODS
  	Stool
  NucleiSENS® EasyMAG® System (BioMérieux)
  MagNA Pure 96 System (Roche)
  QIAmp® DNA Mini Kit (Qiagen)
  QIAsymphony (Qiagen)

  Recommended
  Suitable

  PCR instruments
  Roche	ABI	Bio-Rad	Qiagen
  LC 480	7500 Fast	CFX96	Rotor-Gene
  LC 2.0		DX

  Recommended
  Suitable

• Documents

  SDS	DDGG-40-L100	SDS-DDGG-40-EN-v02-G-DiaShig V02	EN	Download
  SDS	DDGG-40-L100	SDS-DDGG-40-FR-v02-G-DiaShig V02	FR	Download

  CoA	DDGG-40-L100	16216F		Download
  CoA	DDGG-40-L100	2018B08/I	2018-02-26	Download