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Shigella

G-DiaShig™

G-DiaShig
DDGG-40-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DiaControlDNA
YD DR TR Cy5 RD
DiaInhib™
YD DR Cy5

Shigella is a genus of Gram negative, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. Shigella species are classified by four serogroups (A, B, C and D). The causative agent of human shigellosis, Shigella causes disease in primates, but not in other mammals. Shigella is one of the leading bacterial causes of diarrhea worldwide, causing an estimated 80-165 million cases. Shigella infection is typically by ingestion. Shigella causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7. Both Shiga toxin and verotoxin are associated with hemolytic uremic syndrome.

G-DiaShigTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit allowing the qualitative detection of Shigella species (eg, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Shigella boydii) by real-time PCR in stool specimens obtained from patients with signs and symptoms of gastroenteritis.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Shigella FAM 520 nm ipaH 250 µL
    EXTRACTION METHODS
    Stool
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAmp® DNA Mini Kit (Qiagen)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    LC 2.0 DX
    Recommended
    Suitable
  • Documents
    SDS-DDGG-40-EN-v02-G-DiaShig en Download
    SDS-DDGG-40-FR-v02-G-DiaShig fr Download
    CoA_DDGG-40-L100_16216F  Download
  • Publications

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