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Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum

G-DiaPara™

G-DiaPara
DDGG-25-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DiaControlDNA
Cy5 RD
DiaInhib™
Cy5

This panel has been designed to detect three different major parasites in gastronteritis : Entamoeba histolytica, Giardia lamblia and Cryptosporidium parvum

Entamoeba histolytica is an anaerobic parasitic protozoan, part of the genus Entamoeba. Infection can lead to amoebic dysentery or amoebic liver abscess. Symptoms can include fulminating dysentery, bloody diarrhea, weight loss, fatigue, abdominal pain, and amoeboma. The amoeba can actually bore into the intestinal wall, causing lesions and intestinal symptoms, and it may reach the blood stream. 

Giardia lamblia is a flagellated protozoan parasite that colonises and reproduces in the small intestine, causing giardiasis. The giardia parasite attaches to the epithelium by a ventral adhesive disc, and reproduces via binary fission. Giardiasis does not spread via the bloodstream, nor does it spread to other parts of the gastro-intestinal tract, but remains confined to the lumen of the small intestine. 

Cryptosporidium parvum is one of several species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal tract. Primary symptoms of C. parvum infection are acute, watery, and non-bloody diarrhea. C. parvum infection is of particular concern in immunocompromised patients, where diarrhea can reach 10–15 L per day. Other symptoms may include anorexia, nausea/vomiting and abdominal pain.

G-DiaParaTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Entamoeba histolytica, Giardia lamblia & Cryptosporidium parvum in biological samples (stool) collected from patients showing symptoms related to this pathogen using real-time PCR.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Giardia lamblia (intestinalis) FAM 520 nm 18S rRNA 250 µL
    Entamoeba histolytica yellow dye 549 nm 18S rRNA 250 µL
    Cryptosporidium parvum Texas Red 603 nm Segment A 250 µL
    EXTRACTION METHODS
    Stool
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAsymphony (Qiagen)
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    DX
    Recommended
    Suitable
  • Documents
    SDS-DDGG-25-EN-v01-Dia-GE-Parasite-.I-050 en Download
    SDS-DDGG-25-FR-v01-Dia-GE-Parasite-.I-050 fr Download
    DIA-GE_Parasite_I-050_08005C  Download
  • Publications

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