Probes & Primers
RNA extraction control virus
RNA master mix
Norovirus is a nonenveloped RNA virus that causes approximately 90% of epidemic non-bacterial outbreaks of gastroenteritis around the world, and may be responsible for 50% of all foodborne outbreaks of gastroenteritis in the US. Norovirus affects people of all ages. The viruses are transmitted by faecally contaminated food or water, by person-to-person contact and via aerosolization of the virus and subsequent contamination of surfaces. The main symptom is acute gastroenteritis that develops between 12 and 48 hours after exposure. The disease is usually self-limiting, and characterized by nausea, forceful vomiting, watery diarrhea, and abdominal pain, and in some cases, loss of taste. General lethargy, weakness, muscle aches, headache, coughs, and low-grade fever may occur.
Rotavirus is a genus of double-stranded RNA virus in the family Reoviridae. Rotavirus is the most common cause of severe diarrhea among infants and young children, and is one of several viruses that cause infections often called stomach flu, despite having no relation to influenza. By the age of five, nearly every child in the world has been infected with rotavirus at least once. However, with each infection, immunity is developed, subsequent infections are less severe, and adults are rarely affected. There are five species of this virus, referred to as A, B, C, D, and E. Rotavirus A, the most common, causes more than 90% of infections in humans.
G-DiaNotaVTM will be validated on geneLEAD VIII allowing the qualitative detection and differentiation of Norovirus (genogroups I and II) and Rotavirus by real-time PCR in clinical specimens (liquid and soft stools) from patients potentially presenting with gastro-enteric symptoms.