Dientamoeba fragilis

G-DiaFrag™

Dientamoeba fragilis is a one-cell flagellate parasite found in the gastrointestinal tract of some humans, pigs and gorillas. In some people, D. fragilis infection may remain asymptomatic, but generally causes severe gastrointestinal symptoms. It is a major cause of traveler’s diarrhea, chronic diarrhea, abdominal cramping and loss of appetite. Dientamoeba fragilis was recently considered to be more prevalent than Giardia, thus leading to better diagnostics.

G-DiaFrag^TM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Dientamoeba fragilis in biological samples (stool) collected from patients showing symptoms related to this pathogen using real-time PCR.

• Validation

  DOUBLE-DYE PROBE & PRIMERS
  Targeted pathogens	Emission	Targeted genes	Volume
  Dientamoeba fragilis	FAM 520 nm	5,8S rRNA	250 µL

  EXTRACTION METHODS
  	Stool
  NucleiSENS® EasyMAG® System (BioMérieux)
  MagNA Pure 96 System (Roche)
  QIAsymphony (Qiagen)

  Recommended
  Suitable

  PCR instruments
  Roche	ABI	Bio-Rad	Qiagen
  LC 480	7500 Fast	CFX96	Rotor-Gene
  LC 2.0		DX

  Recommended
  Suitable

• Documents

  SDS	DDGG-20-L100	SDS-DDGG-20-EN-v01-Dia-DF-050 V01	EN	Download
  SDS	DDGG-20-L100	SDS-DDGG-20-FR-v01-Dia-DF-050 V01	FR	Download

  CoA	DDGG-20-L100	06005B		Download
  CoA	DDGG-20-L100	02007F	2017-08-30	Download
  CoA	DDGG-20-L100	03008G	2018-11-07	Download
  CoA	DDGG-20-L100	01006J	2017-01-23	Download
  CoA	DDGG-20-L100	2021F15/I	2023-06-01	Download