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Clostridium difficile

G-DiaDiff™

G-DiaDiff
DDGG-30-L100
Probes & Primers
H2O
Positive control
Negative control
100 TESTS
Internal control suggested
DiaControlDNA
DR TR Cy5 RD
DiaInhib™
Cy5

Clostridium difficile is a species of Gram positive bacteria of the genus Clostridium that causes diarrhea and other intestinal diseases when competing bacteria are wiped out by antibiotics. C. difficile is transmitted from person to person by the fecal-oral route. The organism forms heat-resistant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning, thus, these spores survive in clinical environments for long periods. C. difficile may become established in the human colon (2–5% of the adult population). Sometimes antibiotic therapy for various infections has the adverse effect of disrupting the normal balance of the gut flora, in which case Clostridium difficile may opportunistically dominate, causing Clostridium difficile infection. Latent symptoms often mimic some flu-like symptoms. Often, it can be cured simply by discontinuing the responsible antibiotics.

G-DiaDiffTM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Clostridium difficile (tcdA/tcdB) in biological samples (stool) collected from patients showing symptoms related to this pathogen using real-time PCR.

  • Validation
    DOUBLE-DYE PROBE & PRIMERS
    Targeted pathogens Emission Targeted genes Volume
    Clostridium difficile FAM 520 nm tcdA (enterotoxin A) 250 µL
    Clostridium difficile Yellow dye 549 nm tcdB (enterotoxin B) 250 µL
    EXTRACTION METHODS
    Stool
    NucleiSENS® EasyMAG® System (BioMérieux)
    MagNA Pure 96 System (Roche)
    QIAsymphony (Qiagen)
    Recommended
    Suitable
    PCR instruments
    Roche ABI Bio-Rad Qiagen
    LC 480 7500 Fast CFX96 Rotor-Gene
    DX
    Recommended
    Suitable
  • Documents
    SDS-DDGG-30-EN-v02-G-DiaDiff en Download
    SDS-DDGG-30-FR-v02-G-DiaDiff fr Download
    CoA_DDGG-30-L100_16176D-1  Download
  • Publications

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