Clostridium difficile

G-DiaDiff™

Clostridium difficile is a species of Gram positive bacteria of the genus Clostridium that causes diarrhea and other intestinal diseases when competing bacteria are wiped out by antibiotics. C. difficile is transmitted from person to person by the fecal-oral route. The organism forms heat-resistant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning, thus, these spores survive in clinical environments for long periods. C. difficile may become established in the human colon (2–5% of the adult population). Sometimes antibiotic therapy for various infections has the adverse effect of disrupting the normal balance of the gut flora, in which case Clostridium difficile may opportunistically dominate, causing Clostridium difficile infection. Latent symptoms often mimic some flu-like symptoms. Often, it can be cured simply by discontinuing the responsible antibiotics.

G-DiaDiff^TM is a trusted and easy-to-use molecular diagnostic CE-IVD kit designed to qualitatively detect Clostridium difficile (tcdA/tcdB) in biological samples (stool) collected from patients showing symptoms related to this pathogen using real-time PCR.

• Validation

  DOUBLE-DYE PROBE & PRIMERS
  Targeted pathogens	Emission	Targeted genes	Volume
  Clostridium difficile	FAM 520 nm	tcdA (enterotoxin A)	250 µL
  Clostridium difficile	Yellow dye 549 nm	tcdB (enterotoxin B)	250 µL

  EXTRACTION METHODS
  	Stool
  NucleiSENS® EasyMAG® System (BioMérieux)
  MagNA Pure 96 System (Roche)
  QIAsymphony (Qiagen)

  Recommended
  Suitable

  PCR instruments
  Roche	ABI	Bio-Rad	Qiagen
  LC 480	7500 Fast	CFX96	Rotor-Gene
  		DX

  Recommended
  Suitable

• Documents

  SDS	DDGG-30-L100	SDS-DDGG-30-EN-v02-G-DiaDiff V02	EN	Download
  SDS	DDGG-30-L100	SDS-DDGG-30-FR-v02-G-DiaDiff V02	FR	Download

  CoA	DDGG-30-L100	16176D-1		Download
  CoA	DDGG-30-L100	2018E02/I	2018-07-23	Download
  CoA	DDGG-30-L100	2019D16/I	2019-05-03	Download
  CoA	DDGG-30-L100	2020H14/1	2022-08-01	Download