Diagenode Diagnostics offers today an exclusive RNA extraction kit validated on KingFisher™ instruments on more than 20.000 samples, daily used by the National Reference Center for SARS-CoV-2.
This RNA extraction kit (DEXR-10-L100) is based on proprietary magnetic beads and designed for rapid high-throughput purification of SARS-CoV-2 RNA from nasopharyngeal swab samples in 96- well plates (not included).
These reagents (see Table 1) use an optimized method for disrupting samples (50 μL) in a guani- dine thiocyanate-based solution that rapidly releases SARS-CoV-2 RNA while simultaneously in- activating nucleases in the sample matrix. Proprietary magnetic beads with a nucleic acid binding surface are then added to the sample to bind nucle- ic acids. The beads/nucleic acids are captured on magnets, and proteins and other contaminants are washed away. The beads are then washed again to remove remaining binding solution. Nucleic acids are eluted in 50 μL of elution buffer. This protocol is fully automated on KingFisher™ instruments to provide the RNA extraction of up to 96 samples in 45 minutes.
|1||Lysis Buffer||17,50 mL|
|2||RNA Binding Beads||2,70 mL|
|3||Proteinase K||1,20 mL|
|4||Washing Buffer 1||67,00 mL|
|5||Elution Buffer||5,50 mL|
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particu- lates in samples. Since only a small volume of magnetic beads is needed for high-efficiency bind- ing, the bound RNA can be eluted in just 50 μL of elution buffer.
Moreover, this RNA extraction kit (DEXR-10-L100) is the first step towards SARS-CoV-2 detection by RT-qPCR in nasopharyngeal swab samples.
|Packaging||Tubes and bottles|
|List Price||400.00 EUR|
We do not commercialize instruments. Plates will be soon available
All contents are listed in the table 1, on the first page of this document.
KingFisher™ Flex and KingFisher™ 96 are compatible.
Yes, this protocol will be available soon via the Manuals webpage.
The delivery is performed in 2 or 3 business days.
The shelf life is 12 months from the manufacturing date.
50 μL of nasopharyngeal swab is necessary to run the extraction.
The reagents contained in the kit must be stored at Room Temperature.
It should work, the laboratory will have to validate these reagents in the routine extraction workflow.
The extraction protocol will be available soon via the Manuals webpage.
No, this kit is for Research Use Only (RUO).
Pipettes, tips, ethanol and isopropanol.