YOU HAVE NOW ACCESS TO HIGH QUALITY RNA EXTRACTION REAGENTS TO SUPPORT
YOUR DAILY LABORATORY ACTIVITIES IN THE CURRENT SARS-COV-2 PANDEMIC.

Diagenode Diagnostics offers today an exclusive RNA extraction kit validated on KingFisher™ instruments on more than 20.000 samples, daily used by the National Reference Center for SARS-CoV-2.

This RNA extraction kit (DEXR-10-L100) is based on proprietary magnetic beads and designed for rapid high-throughput purification of SARS-CoV-2 RNA from nasopharyngeal swab samples in 96- well plates (not included).

These reagents (see Table 1) use an optimized method for disrupting samples (50 μL) in a guani- dine thiocyanate-based solution that rapidly releases SARS-CoV-2 RNA while simultaneously in- activating nucleases in the sample matrix. Proprietary magnetic beads with a nucleic acid binding surface are then added to the sample to bind nucle- ic acids. The beads/nucleic acids are captured on magnets, and proteins and other contaminants are washed away. The beads are then washed again to remove remaining binding solution. Nucleic acids are eluted in 50 μL of elution buffer. This protocol is fully automated on KingFisher™ instruments to provide the RNA extraction of up to 96 samples in 45 minutes.

Table 1. Kit Contents

Buffer	Description	Volume
1	Lysis Buffer	17,50 mL
2	RNA Binding Beads	2,70 mL
3	Proteinase K	1,20 mL
4	Washing Buffer 1	67,00 mL
5	Elution Buffer	5,50 mL

Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particu- lates in samples. Since only a small volume of magnetic beads is needed for high-efficiency bind- ing, the bound RNA can be eluted in just 50 μL of elution buffer.

Moreover, this RNA extraction kit (DEXR-10-L100) is the first step towards SARS-CoV-2 detection by RT-qPCR in nasopharyngeal swab samples.

Table 2. Kit Specifications

Contents	5 Buffers
Extractions	100
Packaging	Tubes and bottles
Reference	DEXR-10-L100
List Price	400.00 EUR

FAQ

1. Would it be possible to purchase consumables (plates...) and instrument at Diagenode Diagnostics?

We do not commercialize instruments. Plates will be soon available

2. What is included in this extraction reagent kit?

All contents are listed in the table 1, on the first page of this document.

3. Which KingFisher instruments are compatible with this extraction reagents?

KingFisher™ Flex and KingFisher™ 96 are compatible.

4. Is a specific protocol required to run an extraction with these reagents?

Yes, this protocol will be available soon via the Manuals webpage.

5. How long does it take for reagents delivery?

The delivery is performed in 2 or 3 business days.

6. What is the shelf life of this extraction reagent kit?

The shelf life is 12 months from the manufacturing date.

7. What is the starting material required for this extraction?

50 μL of nasopharyngeal swab is necessary to run the extraction.

8. What are the storage conditions of this extraction reagents?

The reagents contained in the kit must be stored at Room Temperature.

9. Are other types of specimen compatible with this extraction reagent kit?

It should work, the laboratory will have to validate these reagents in the routine extraction workflow.

10. What is the extraction protocol?

The extraction protocol will be available soon via the Manuals webpage.

11. Is this extraction reagent kit CE-IVD marked?

No, this kit is for Research Use Only (RUO).

12. Which additional material is necessary to perform the extraction?

Pipettes, tips, ethanol and isopropanol.

13. How many extractions can be performed with this kit?

100 extractions.

Feel free to contact your local Diagenode Diagnostics representative or our customer support by email info@diagenodediagnostics.com or by phone +32 4 290 49 39 for any additional information related to these products.