Diagenode Molecular Diagnostics

Diagenode controls

Diagenode Diagnostics assays can be used with 3 different type of controls.

DiaControlDNA™ & DiaControlRNA™:
Diagenode PCR Extraction and Inhibition Control for for Real-Time PCR, DNA/RNA pathogens

DNA or RNA virus having no sequences similarities with human genome or with pathogens affecting human. These extraction and inhibition controls allow users of diagnostic assays to asses nucleic acid extraction efficiency and detect the presence of PCR inhibitor or process error.

DNA

YD Yellow Dye : ref DICD-YD-L100
DR Dragon Dye : ref DICD-DR-L100
TR Texas Red : ref DICD-TR-L100
Cy5 Cy5 : ref DICD-CY-L100
RD Red Dye : ref DICD-RD-L100

RNA

YD Yellow Dye : ref DICR-YD-L100
DR Dragon Dye : ref DICR-DR-L100
TR Texas Red : ref DICR-TR-L100
Cy5 Cy5 : ref DICR-CY-L100
RD Red Dye : ref DICR-RD-L100

RNA 100X (RNA EIC with a 100X concentrated viral culture, to be used with RNA pathogens respiratory PCR kits)

YD Yellow Dye DRIC-YD-L100
Cy5 Cy5 : ref DRIC-CY-L100

DiaInhib™ : Diagenode PCR Inhibition Control for Real-Time PCR

This control is a plasmid that has no sequence similarity with human genome or with pathogens affecting human. This universal inhibition control enables users of diagnostic assays to detect the presence of PCR inhibitor or process error.

YD Yellow Dye : ref DICU-YD-L100
DR Dragon Dye : ref DICU-DR-L100
Cy5 Cy5 : ref DICU-CY-L100

FAM Yellow Dye Orange Dragon Fly Texas Red Cy5/Red Dye
ABI 7300 FAM VIC NED Rox
ABI 7500 Fast FAM VIC NED Texas Red Cy5 Cy5
ABI 7900 FAM VIC NED Rox
Roche LightCycler® 2.0 530 560 Rox
Roche LightCycler® 480 465/510 533/580 533/610 618/670 618/670
Roche Cobas® 4800 465/510 540/580 540/610 618/670
Bio-Rad CFX96™ FAM HEX Texas Red Cy5 Cy5
Qiagen Rotor-Gene® 3000 FAM JOE Rox Cy5 Cy5
Qiagen Rotor-Gene® Q 6000 Green Yellow Orange Red Red
Stratagene Mx 3000P™ FAM HEX Texas Red Cy5 Cy5
Stratagene Mx 3005P™ FAM HEX Texas Red Cy5 Cy5
FAM YD DR TR Cy5 RD ***
Excitation 494 530 554 583 646 644
Emission (nm) 520 548 576 603 662 669
DICD-YD-L100
DICR-YD-L100
DICU-YD-L100
DICD-DR-L100
DICR-DR-L100
DICU-DR-L100
DICD-TR-L100
DICR-TR-L100
DICD-CY-L100
DICR-CY-L100
DICU-CY-L100
DICD-RD-L100
DICR-RD-L100

* FAM available for LC 2.0
** DR suggested for ABI PCR System
*** RD especially for Cepheid Smart Cycler instead of CY5

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