R-DiaTub

DDGR-70-L100
(Dia-MT-050)

Mycobacterium tuberculosis (MTB) is a pathogenic bacterial species of the genus Mycobacterium and the causative agent of most cases of tuberculosis.

Tuberculosis is a common and severe infectious disease that usually attacks the lungs but can also affect the central nervous system, the lymphatic system, the circulatory system, the genitourinary system, the gastrointestinal system, bones, joints, and even the skin.

Other mycobacteria such as Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canettiMycobacterium microti also cause tuberculosis, but these species are less common.


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PRODUCT INFORMATION

Researched pathogens • Mycobacterium tuberculosis: FAM dye, emission: 520 nm
Target genes • IS6110 (Mycobacterium tuberculosis)
Number of wells by test 1 (Mycobacterium tuberculosis)
Samples used • Bronchoalveolar lavage
• Sputum
Extraction methods • Nuclisens easyMAG® System (Biomerieux)
• QIAamp® DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen)
• MagNa pure LC system (Roche) (Ongoing)
Real-time PCR systems • ABI (7000/7300/7500/7900)
• Roche LightCycler® (2.0/480)
• Biorad (iCycler/IQ5/CFX96/Dx)
• Qiagen Rotor-Gene (6000)
• Stratagene (MX3000P/3005P)
• Cepheid (SmartCycler II)
PCR volumes 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)
PCR reactions / kit • 50 reactions (50 µl PCR, final volume)
• 100 reactions (25 µl PCR, final volume)
• 120 reactions (20 µl PCR, final volume)
In combination with DiaInhib:
DiaInhib(YD): yellow dye, emission: 549 nm
DiaInhib(DR): orange dye, emission: 576 nm
DiaInhib(TR): texas red dye, emission: 603 nm
DiaInhib(Cy5): Cy5 dye, emission: 662 nm

DiaControlDNA:
DiaControlDNA(YD): yellow dye, emission: 549 nm
DiaControlDNA(DR): orange dye, emission: 576 nm
DiaControlDNA(TR): texas red dye, emission: 603 nm
DiaControlDNA(Cy5): Cy5 dye, emission: 662 nm