Trichomonas vaginalis is an anaerobic, flagellated protozoan, a form of microorganism. The parasitic microorganism is the causative agent of trichomoniasis, and is the most common pathogenic protozoan infection of humans in industrialized countries (1). Infection rates between men and women are the same with women showing symptoms while infections in men are usually asymptomatic. Transmission takes place directly because the trophozoite does not have a cyst Product.

(1) Soper D (January 2004). "Trichomoniasis: under control or undercontrolled?". American journal of obstetrics and gynecology 190 (1): 281–90

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Researched pathogens Trichomonas vaginalis: FAM dye, emission: 520 nm
Target genes Repeated sequence
Number of wells by test 1 (Trichomonas vaginalis)
Samples used • urine
• swab
Extraction methods • Nuclisens EASYMAG System (Biomerieux)
• QIAamp DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen)
Real-time PCR systems • ABI (7000/7300/7500/7900)
• Roche lightCycler (2.0/480)
• Biorad (ICycler/IQ5/CFX96/Dx)
• Qiagen RotorGene (6000)
• Stratagene (MX3000P/3005P)
• Cepheid (SmartCycler II)
PCR volumes 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)
PCR reactions / kit • 50 (50 µl PCR)
• 100 (25 µl PCR)
• 120 (20 µl PCR)
In combination with DiaInhib:
DiaInhib(YD): yellow dye, emission: 549 nm
DiaInhib(DR): orange dye, emission: 576 nm
DiaInhib(Cy5): Cy5 dye, emission: 662 nm

DiaControlDNA(YD): yellow dye, emission: 549 nm
DiaControlDNA(DR): orange dye, emission: 576 nm
DiaControlDNA(Cy5): Cy5 dye, emission: 662 nm