H-DiaCMVQ

DDGH-10-L200
(Dia-CMVQ-050)

Cytomegalovirus, ds DNA enveloped virus, is a member of the herpesvirus family. These viruses have the ability to remain alive, but dormant, in the body for life.

Cytomegalovirus may be shed in the body fluids of any previously infected person, and thus may be found in urine, saliva, blood, tears, semen, and breast milk.

Cytomegalovirus infection is important to certain high-risk groups like unborn babies during pregnancy, people who work with children and immunocompromised patient, such as organ transplant recipients and individuals infected with human immunodeficiency virus (HIV).


Download the manual Last update: April 23, 2014

Télécharger le manuel d'utilisation Last update: April 23, 2014

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PRODUCT INFORMATION

Researched pathogens • Cytomegalovirus: FAM dye, emission: 520 nm
Target genes • Glycoprotein B (Cytomegalovirus)
Number of wells by test 1 (Cytomegalovirus) + 5 for the CMV quantitative curve
Samples used • Whole blood
• Plasma
Extraction methods • Nuclisens easyMAG® System (Biomerieux)
• QIAamp® DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen)
• Maxwell 16 Blood DNA Purification kit (48) (Promega)
Real-time PCR systems • ABI (7000/7300/7500/7900)
• Roche LightCycler® (2.0/480)
• Biorad (iCycler/IQ5/CFX96/Dx)
• Qiagen Rotor-Gene (6000)
• Stratagene (MX3000P/3005P)
PCR volumes 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)
PCR reactions / kit • 50 reactions (50 µl PCR, final volume) + 5 CMV quantitative curves
• 100 reactions (25 µl PCR, final volume) + 5 CMV quantitative curves
• 120 reactions (20 µl PCR, final volume) + 5 CMV quantitative curves
In combination with DiaInhib:
DiaInhib(YD): yellow dye, emission: 549 nm (included in the kit)
DiaInhib(DR): orange dye, emission: 576 nm
DiaInhib(Cy5): Cy5 dye, emission: 662 nm

DiaControlDNA:
DiaControlDNA(YD): yellow dye, emission: 549 nm
DiaControlDNA(DR): orange dye, emission: 576 nm
DiaControlDNA(Cy5): Cy5 dye, emission: 662 nm